果胶酶高产菌株EIM-4的鉴定及其液体发酵条件优化

Identification of A Pectinase-producing Fungi EIM-4 and Its Orthogonal Experiment of Submerge Fermentation Conditions

目的:通过了解高产果胶酶菌株EIM-4的背景信息及优化其最适的产酶条件,为下一步工业化生产提供依据。方法:通过基于18S rDNA序列的系统发育进化分析对果胶酶高产菌进行鉴定,通过单因素试验和均匀设计获得最适产酶条件。结果:通过对18SrRNA基因的分子系统进化分析,菌株EIM-4被鉴定为黑曲霉(Aspergillus niger)。通过单因素和均匀设计试验确定最佳产酶培养基为(g/L):橘皮粉32.9,黄豆粉10.7,(NH4)SO42.1,CaCO31.0,pH自然。结论:Aspergillus niger EIM-4的液体发酵产果胶酶的活力可达15159.82U/mL。

Objective：Developing available industrial technologies to produce pectinase by understanding basic information for a high pecfinaseproducing strain EIM4 and optimizing its submerged fermentation conditions. Method： Identified strain EIM4 by using molecular phyloganatic analysis of 18S rDNA sequences and improved pectinase production by orthogonal experiment design. Result：Strain ElM- 4 was identified as Aspergillus ruiger. Through orthogonal experiment, the optimal submerge fermentation condition was obtained for producing extracellular pectinase. suitable medium contained： orange peel power 32.97g/L, soybean flour 10.76g/L, （NIL）SO4 2.14g/L, CaCO3 1.00g/L., nature pH. Condusion：The optimum pectinase activity of Aspergillus niger EIM-4 is as high as 15 159.82U/ml.