DNA倍体图像分析用于评价宫颈非典型不成熟化生

Evaluation of cervical atypical immature metaplasia by DNA computerized image analysis

目的:探讨DNA图像分析技术在评价宫颈非典型不成熟化生(AIM)生物学性质中的作用。方法:运用计算机图像分析软件测定宫颈非典型不成熟化生30例的DNA质量、非整倍体(AN)含量、DNA指数(DI),以40例良、恶性宫颈病变为对照,其中正常上皮组10例,CINⅠ组10例,CINⅢ组10例,宫颈鳞癌组10例。结果:DNA质量、非整倍体含量、DNA指数随病变严重程度的增加而升高,三者联合分析有助于评价病例的生物学性质,从30例宫颈非典型不成熟化生病例中筛选出2例,其DNA质量、非整倍体含量、DNA指数均达到CINⅢ组的均值水平,认为是高恶性潜能病例。结论:计算机分析技术方便客观,通过综合分析DNA质量、非整倍体含量和DNA指数有助于提示宫颈非典型不成熟化生病例的生物学性质,对高恶性潜能病例具有筛查作用。

Objective： To evaluate the effect of DNA computerized image analysis in biological behavior describing and prognosis prediction of cervical atypical immature metaplasia （AIM）. Methods： Average DNA contents, DNA aneuploid, DI by computerized image software were measured in 30 cases of AIM, 10 cases of normal/mature metaplasia cervical tissues, 10 cases of CIN Ⅰ , 10 cases of CIN m, 10 cases of cervical squamous carcinoma. Results：According to the changes of those 3 indexes with the pathological progressing, 2 cases of high-grade AIM were detected. Conclusion： DNA computerized image analysis is useful and objective in reflecting the biological behavior and in screening high-grade AIM.