两种84消毒液临床实际使用消毒效果比较

COMPARISON OF DISINFECTION EFFICACY OF TWO KINDS OF 84 DISINFECTION SOLUTION IN CLINICAL ACTUAL USE

目的观察普通84消毒液与含碘84消毒液在临床实际使用消毒效果,以规范临床使用方法。方法采用悬液定量杀菌试验和理化分析方法进行了杀菌效果和有效成分含量测定,同时用酶联免疫吸附法测定其对表面抗原破坏作用。结果两种84消毒液均随着浸泡物品时间延长,其有效氯下降率增加;以普通84消毒液下降速度更快。用含有效氯1000 mg/L的普通84消毒液对悬液内枯草杆菌黑色变种芽孢作用90 min,平均杀灭对数值均>5.0;含碘84消毒液在相同条件下作用60 min,平均杀灭对数值亦达到5.05;使用24 h之后,杀菌效果下降。用有效氯1000 mg/L的两种84消毒液作用15 min,均可完全灭活悬液内HBsAg活性。结论两种84消毒液均可有效杀灭细菌芽孢和破坏乙型肝炎表面抗原,含碘84消毒液杀菌效果略好于普通84消毒液。

Objective To observe the disinfection efficacy of ordinary 84 disinfection solution and the 84 disinfection soluton containing iodine in clinical actual use in order to standardize clinical application method. Methods Suspension quantitative germicidal test and physicochemical analysis method were used to determine the germicidal efficacy and the active principle content. ELISA was used to determine the destructive effect on HBsAg. Results The rate of decrease in available chlorine content in the 2 kinds of 84 disinfection solution increased with prolonging the immersion disinfection time, especially the rate of decrease in the ordinary 84 disinfection solution was quicker. The average killing logarithm value of spores of Bacillus subtilis var. niger in suspension exposed to the ordinary 84 disinfection solution containing available chlorine 1000 mg/L for 90 min was 〉5.0, and that exposed to the 84 disinfection solution containing iodine for 60 min under same conditions was 5.05. After use for 24 h, the germicidal efficacy decreased. Both 84 disinfection solutions containing available chlorine 1000 mg/L with a contact time of 15 min could completely inactivate HBsAg activity in suspension. Conclusions Both 84 disinfection solutions can effectively kill bacterial spores and destroy HBsAg. The germicidal efficacy of the 84 disinfection solution containing iodine is slightly better than that of the ordinary 84 disinfectiion solution.