β-环糊精对双酚A分子识别作用的表征研究

Study on characterization of molecular recognition interaction of bisphenol A by β-cyclodextrin

运用紫外光谱、红外光谱、荧光光谱、H核磁共振谱研究了β-环糊精对双酚A的分子识别行为,结果表明：双酚A经分子识别后其紫外吸收有所增加,吸收峰发生2～3 nm红移;红外特征峰被β-环糊精不同程度掩盖,且特征吸收峰都在不同程度发生一定的偏移;荧光强度随β-CD浓度的增大而逐渐增强;β-环糊精与双酚A形成稳定1∶1的包结物,包结物常数为1.84×10^4L.mol^-1,双酚A从β-环糊精的大口端部分进入其空腔.

Molecular recognition behavior of bisphenol A by β-cyclodextrin was studied with ultraviolet spectrum, infrared spectrum, fluorescence spectrum and ^1HN MR, the results from ultraviolet spectrum showed that the absorbance of bisphenol A after molecular recognition increases with increasing of the concentration of β-cyclodextrin and results in 2--3 nm red shift of maximum absorbance wavelength, the results from infrared spectrum showed that some characteristic peaks of bisphenol A after molecular recognition were covered and results in shift of absorbance peaks, the results from fluorescence spectrum showed that the fluorescence intensity of bisphenol A after molecular recognition increases with increasing of the concentration of β-cyclodex- trin.β-cyclodextrin reacts with bisphenol A forming a 1 ： 1 inclusion complex, the formation constant of which is 1.84 × 10^4 L·mol^-1 ,bisphenol A can enter the inner cavity of β- CD from the bigger opening.