摘要
采用HPLC法同时测定复方磺胺间甲氧嘧啶钠溶液中磺胺间甲氧嘧啶钠和甲氧苄啶的含量。色谱柱为Kromasil C18柱(250 mm×4.6 mm,粒径5μm),流动相为0.02 mol/L磷酸溶液-甲醇(80∶20,V/V),检测波长270 nm,流速1.0 mL/min,柱温30℃。在10~500μg/mL浓度范围内,磺胺间甲氧嘧啶钠和甲氧苄啶均呈线性关系。平均回收率分别为99.25%和99.76%。该法准确可行,适用于同时检测复方磺胺间甲氧嘧啶钠溶液中磺胺间甲氧嘧啶钠和甲氧苄啶的含量。
An HPLC method has been developed to determine sulfamonomethoxine sodium and trimethoprim in compound sulfamonomethoxine sodium solution. Using Kromasil C18 column(250 mm ×4.6 mm, 5 μm) with a mobile phase of 0.02 mol/L phosphoric acid solution - methanol(80: 20, V/V) at the wavelength of 270 nm. The flow rate was 1.0 mL/min and the colum temperature was kept at 30 ℃. Both sulfamonomethoxine sodium and trimethoprim were linear within the range of 10 - 500 μg/mL. The average recoveries were 99.25% and 99.76%, respectively. The method is accurate and feasible and can be used for simultaneous determination of sulfamonomethoxine sodium and trimethoprim in compound sulfamonomethoxine sodium solution.
出处
《中国兽药杂志》
2008年第11期27-29,共3页
Chinese Journal of Veterinary Drug