摘要
In this article, a improved PCR method of detection of minimal residual disease (MRD) was reported, in which, the DNA templates for PCR amplification were extracted from bone marrow smears. The course of DNA extraction was relatively simple and time-saving. Of 60 cases, adequate DNA was obtained in 57 cases. The DNA from 2 to 3 slides could be used for 3 to 4 PCR amplifications. With this method, we detected MRD in 9 children with acute lymphoblastic leukemia after therapy. 4 out of the 9 patients were MRD-positive, and 2 MRD-positive patients relapsed soon after detection, otherwise, the 5 MRD-negative patients didn’t relapse. We suggest that this improved MRD-PCR method could be widelyused in clinical practice.
In this article, a improved PCR method of detection of minimal residual disease (MRD) was reported, in which, the DNA templates for PCR amplification were extracted from bone marrow smears. The course of DNA extraction was relatively simple and time-saving. Of 60 cases, adequate DNA was obtained in 57 cases. The DNA from 2 to 3 slides could be used for 3 to 4 PCR amplifications. With this method, we detected MRD in 9 children with acute lymphoblastic leukemia after therapy. 4 out of the 9 patients were MRD-positive, and 2 MRD-positive patients relapsed soon after detection, otherwise, the 5 MRD-negative patients didn't relapse. We suggest that this improved MRD-PCR method could be widelyused in clinical practice.
出处
《中国小儿血液》
1997年第3期114-115,共2页
China Child Blood
