HPLC同时测定三宝胶囊中原儿茶醛、丹参素钠和丹酚酸B含量

RP-HPLC determination of protocaechuic aldehyde sodium Danshensu and salvianolic acide B in Sanbao capsules

目的：建立HPLC法同时测定三宝胶囊中原儿茶醛、丹参素钠、丹酚酸B含量。方法：以三宝胶囊为研究对象，采用Hypersil BDS C18（5μm，250×4．6mm）色谱柱；流动相A：含0．05％磷酸的甲醇溶液，B：0.05％磷酸水溶液，梯度洗脱。流速1mL·min^-1；检测波长270nm。结果：原儿茶醛、丹参素钠、丹酚酸B分别在0．0248-0．248μg（r=0.9995）、0．370-3．70μg（r=0．9999）和0．252-2．52μg（r=0．9998）范围内呈良好的线性关系；平均回收率依次为：99．3，98．6，98．8。结论：该法操作简便、快速、准确，为三宝胶囊质量评价提供了可靠方法。

Objective： To develop a method to determine protocaechuic aldehyde, sodium Danshensu and salvianolic acide B in Sanbao capsules. Methodes： Using RP-HPLC method with Hypersil BDS C 18 （5μm, 250mm×4.6mm） column, the mobile phase A was methanol （ 0.05 % phosphoric acid ）, B was water （0.05% phosphoric acid） eluted in gradient mode. Flow rate was 1mL·min^-1, the detetion wavelength was 270nm,and column temperature was 35℃. Results： The calibration curves were linear in the range of 0.0248μg-0.248μg（ r = 0.9995 ） for protocaechuie aldehyde, 0.3701μg- 3. 70μg（ r = 0.9999） for sodium Danshensu and 0.252μg-2.52μg （ r = 0.9998 ） for salvianolic acide B. The average recovery of protocaeehuic aldehyde was 99.3% ,of sodium Danshensu was 98.6% and of salvianolic acide B was 98.8% . Conclusion ：The method is convenient and appropriate and can be applied to the quality control of Sanbao capsules.