板蓝根抗病毒有效部位筛选

Screening of the Antiviral Active Parts from Isatis indigotica

目的:对板蓝根药材中提取的10个化学部位进行体外抗单纯疱疹病毒(HSV)活性研究,确定板蓝根抗病毒的有效部位。方法:利用溶剂法和色谱法从板蓝根中提取分离出10个粗提和精制部位,用MTT法检测这10个部位体外对HSV-Ⅰ及HSV-Ⅱ型病毒的抑制作用,以病毒抑制率和治疗指数(TI)为评价指标,比较各化学部位体外抗病毒的效果。结果:水提大孔树脂吸附的10%、50%醇洗部位(Ⅷ、Ⅹ)作用最强,对HSV-Ⅰ型病毒的抑制率为分别为53.21%、56.28%,接近阳性对照药阿昔洛韦(62.55%),其TI分别为4.61、18.62。结论:板蓝根不同化学部位的抗病毒作用强度有较大差异。

OBJECTIVE：To study the activity of 10 chemical parts extracted from Isatis indigotica against herpes simplex virus（HSV） in vitro and to determine the antiviral active parts.METHODS： 10 crude fractions and refined portions were extracted from Isatis indigotica using solvent isolation and absorption chromatography.Their inhibitory effects against HSV- Ⅰ and HSV- Ⅱ were determined by MTT using virus inhibition ratio and therapeutic index（TI） as appraisal targets to compare the antiviral effects among all the chemical parts.RESULTS： The parts（Ⅷ, Ⅹ ） with EtOH（10% and 50% in concentration） elution via macroporous resin absorption showed the most potent activities, whose inhibitory rates against HSV- Ⅰ were 53.21% and 56.28% respectively, similar the action of its positive control acyclovir（62.55% ）, and their TI were 4.61 and 18.62 respectively.CONCLUSION： There were significant differences in the antiviral activity among all the chemical parts extracted from Isatis indigotica.