思的明胶囊提取工艺研究

Study on Extraction Technique of Sidiming Capsule

目的:优选思的明胶囊的最佳提取工艺。方法:选用L9(34)正交试验,分别对煎煮和醇沉2个工艺步骤进行优选,以干膏率为煎煮工艺指标,梓醇总提取量为醇沉工艺综合评分指标;采用高效液相色谱法测定梓醇含量。结果:最佳提取工艺为药材加8倍量水,煎煮2次,每次煎煮1h,水提液过滤、合并、浓缩至1.0g(生药).mL-1,再用乙醇沉淀,醇沉浓度为75%,沉淀24h,过滤取上清液,微波真空浓缩干燥得干膏。结论:本方法稳定、合理,可作为思的明胶囊的最佳提取工艺。

OBJECTIVE：To optimize the extraction technique for Sidiming capsules.METHODS：The technical conditions for the water decoction and the alcohol precipitation were optimized respectively by the orthogonal experiment design L9（3^4） with hydrosoluble extract used as the index for the water decoction and the catalpol extract for alcohol precipitation.The content of Catalpol was determined by HPLC. RESULTS： The optimum conditions were as follows： decocting the crude drugs twice with 8-fold water, 1 h each time. The physic liquor extracted by water was filtered, mixed and concentrated to 1.0 g · mL^-1（crude drug）, and then precipitated by 75% concentration of alcohol for 24 h.Then the physic liquor was filtered, concentrated and dried by microwave vacuum concentration dryer to obtain the dry ointment. CONCLUSION： The optimum extraction procedure is stable and reliable, and it can be used as the optimal extraction procedure of Sidiming capsules.