电针抗吗啡戒断后焦虑的5-HT_(1A)受体作用机理研究

Mechanism of Electro-acupuncture on 5-HT1A Receptor in the Anxious Mice after Morphine Withdrawal

目的:探讨电针抗吗啡戒断后焦虑的5-HT1A受体作用机理。方法:建立吗啡依赖戒断小鼠模型,分别予模型小鼠皮下连续注射5-HT1A受体激动剂(8-OH-DPAT)或拮抗剂(WAY-100635),并予电针治疗后,以高架十字迷宫测试小鼠焦虑情绪。结果:(1)电针和8-OH-DPAT均可提高模型小鼠的OT%和OE%值(P<0.05)。(2)电针可明显提高注射WAY-100635的模型小鼠的OT%和OE%值(P<0.05)。结论:5-HT1A受体在抗焦虑过程中起重要作用,它可能是电针抗焦虑的重要中枢作用途径之一。

Objective： To research the mechanism of electro - acupuncture on 5 - HT1A receptor in the anxious mice after morphine withdrawal. Methods： Established a morphine withdrawl mice model, and then observed the model injected 5 -HT1A receptor agonist （8 - OH - DPAT） or antagonist （ WAY - 100635 ） with Elevated Plus - maze after electro - acupuncture treat- ment. Results ： （ 1 ） Both of electro - acupuncture treatment and 8 - OH - DPAT could raise the value of OT% ＆ OE% （ P 〈 0. 05） of the model mice. （2）Electro- acupuncture treatment could raise the value of OT% ＆ OE% （ P 〈 0.05） of the model mice injected WAY - 100635. Conclusion： The 5 - HT1A receptor played a significant role during the antianxiety treatment with electro - acupuncture, and which was possibly one of the primary central agents in the process.