曲美他嗪对大鼠局灶性脑缺血再灌注Bcl-2 mRNA、Caspase-3 mRNA表达的影响

Effects of TMZ on Bcl-2/ Caspase-3 mRNA’s Expression in the Focal Cerebral Ischemia and Reperfusion Models

目的观察曲美他嗪(TMZ)对大鼠局灶性脑缺血后神经细胞Caspase-3 mRNA、Bcl-2 mRNA表达的影响。方法成年健康Wistar大鼠45只,随机分为假手术对照组(n=5)、脑缺血再灌注组(n=20)、TMZ预处理组(n=20)。应用线栓法建立大脑中动脉闭塞再灌注模型。采用原位杂交方法检测脑组织Caspase-3 mRNA、Bcl-2m RNA表达的变化。结果Bcl-2 mRNA在缺血再灌注3h表达增强(P<0.05),6h显著增强(P<0.01),24h减弱(P<0.05),48h消失(P>0.05)。TMZ组的Bcl-2 mRNA表达量多于缺血再灌注组,差异有统计学意义(P<0.05)。Caspase-3 mRNA在再灌注3h表达增强(P<0.05),6h显著增强(P<0.01),24h更加明显(P<0.01),48h减弱(P<0.05)。TMZ组的Caspase-3 mRNA表达量显著少于缺血再灌注组,差异有统计学意义(P<0.05)。结论TMZ可能通过诱导Bcl-2的表达和抑制Caspase-3的生成而发挥抗凋亡作用。

Objectives To observe trimetazidine （TMZ） influence of the Bcl-2 mRNA, Caspase-3 mRNA expression in the focal cerebral ischemia and reperfusion tissue. Methods To make use of the focal cerebral ischemia and reperfusion model induced by a suture method which preferred by Zea Longa in rats,45 adult Wistar rats randomly were allocated to the sham-operation group, ischemia and reperfusion group and TMZ pretreatment group. Hybridization in situ technique was used to investigate the Bcl-2 mRNA, Caspase-3 mRNA. Results （1）The expression of Bcl-2 mRNA：hybridization signals in ischemia-reperfusion group became apparent at the third hour after reperfusion（P〈0.05）, it became significantly apparent at the 6th hour（P〈0.01）, and weak at the 24th hour, and almost disappeared at the 48th hour（P〉0.05）. In TMZ group, the signals became apparent at the third hour, significantly apparent at the 6th hour, and gradually weak from the 24th hour to the 48th hour. Compared in the reperfusion group, the expression of Bcl-2 mRNA in the TMZ group was significantly higher（P〈0.05）. （2）The expression of Caspase-3 mRNA：hybridization signals in the ischemia-reperfusion group became apparent at the 3rd hour after reperfusion, it became significantly apparent at the 6th hour, and further increased at the 24th hour, and decreased at the 48th hour. In the TMZ group, the signals were scattered at the third hour,became apparent at the 6th hour, significantly apparent at the 24th hour, and almost disappeared at the 48th hour （P〉0.05）. Conclusion TMZ can inhibit the activation of Caspase-3 mRNA and promote the expression of Bcl-2m RNA in the cerebral ischemia and reperfusion, inhibit the neuronal apoptosis and protect the brain.