哮喘小鼠Th1/Th2细胞偏移及卡介苗干预的影响

Excursion of Th1/Th2 and Effect of BCG in Asthmatic Mice

目的研究哮喘小鼠Th1/Th2细胞偏移情况,同时观察卡介苗(BCG)对Th1/Th2偏移的影响。方法将雄性昆明小鼠随机分成哮喘组、BCG干预组和对照组。用卵清蛋白(OVA)致敏、激发建立哮喘模型。ELISA法测定肺泡灌洗液、脾细胞培养上清液中IFN-γ、IL-4的水平。结果肺泡灌洗液、脾细胞培养上清液中IFN-γ/IL-4比值在OVA致敏后均明显降低,初次激发后进一步降低,多次激发后继续降低,与对照组相比,差异均有统计学意义(均P<0.05)。BCG干预后IFN-γ/IL-4比值较哮喘组增高,但仍低于对照组,差异有统计学意义(P<0.05)。结论致敏状态下Th1/Th2比例发生偏移,随着抗原激发,Th1/Th2偏移愈加明显,在哮喘气道炎症中,以Th2亢进为主,而代表全身性炎症的脾脏中,则以Th1分泌减少为主。BCG干预后可以部分纠正Th1/Th2偏移,但BCG作用有时限性。

Objectives By observing the production of interferon-gamma（IFN-γ）, interleukin-4（IL-4） in bronehoalveolar iavage fluid （BALF） and spleen mononuclear cell culture supernant, to clarify the changes of Th1/Th2 during the course of asthma. The influence of bacillus ealmette-guerin（BCG） was also investigated in this study. Methods Kunming mice were randomly divided into the asthma group, BCG group and control group.Mice were sensitized and challenged by ovalbumin （OVA） to make sensitive and asthmatic models.The amount of IFN-γ, IL-4 was assessed by ELISA. Results In BALF and spleen mononuelear cell culture supernant, IFN-γ/IL-4 was decreased in the asthma group compared with that in the control group after sensitized and decreased significantly after first challenge and multiple challenges（P〈0.05）. IFN-γ/IL-4 in BCG group increased compared with that in the asthma group after OVA sensitized, but there was no difference compared with the control group,IFN-γ/IL-4 increased after first,multiple challenges, but lower than that in the control group. Conclusion Th1/Th2 is excursed in sensitization. After challenge, excursion of Th1/Th2 is more significant. IFN-γ dominates in the development of airway inflammation, while IL-4 took the major role in systematic inflammation. BCG can retrieve the excursion of Th1/Th2 partly.