uPAR在肝细胞癌中的表达及其和肝癌侵袭、转移的关系

Relationship Between the expression of uPAR and Invasion and Metasitasis of Hepatocellular Carcinoma

目的研究尿激酶型纤溶酶原激活受体(uPAR)在肝癌组织及细胞系中的表达,探讨其与肝细胞癌侵袭、转移的关系。方法利用免疫组化方法检测50例肝癌组织中uPAR的表达,分析其与肝癌的临床病理分级、包膜侵犯、门脉癌栓等的关系;利用流式细胞术检测肝癌细胞系Hep-3B和SMMC-7721中uPAR的表达,通过Boyden小室侵袭实验比较两种细胞系体外侵袭能力的差别;从SMMC-7721中通过流式细胞术分选出uPAR阳性及阴性的细胞,比较两者体外侵袭能力的差别,并通过裸鼠体内成瘤及转移模型,比较两者体内侵袭和转移的能力。结果肝癌中uPAR的表达和肿瘤的包膜侵犯、门脉癌栓有显著的相关性(P<0.01);肝癌细胞系Hep-3B和SMMC-7721中uPAR的表达和其体外侵袭能力相关;而在SMMC-7721中,uPAR阳性组较阴性组有更强的体外及体内侵袭及转移能力。结论uPAR蛋白的表达在肝癌细胞的侵袭和转移中起重要作用,和肿瘤的临床病理分期及预后相关。

Objective To study the expression of urokinase-type plasminogen activator receptor （uPAR） in hepatoeellular carcinoma ,and to investigate the relationship between the expression of uPAR and invasion or metastasis of HCC. Methods By using immunohistoehemistry the expression of uPAR in 50 specimens of HCC was detected, then the clinical and pathological significance of the expression of uPAR was studied; by FACS ,the expression of uPAR in Hep-3B and SMMC-7721 and their invasion in vitro were studied;also by FACS, SMMC-7721 were divided into uPAR positivity and negativity groups ,and their ability of invasion and mestasis in vitro and in vivo were studied. Results The expression of uPAR in HCC was higher than that in the normal and the paratumor fissure, and had significant correlation with capsule invaded and tumor thrombi in portal vein in HCC （P〈0.01）. In HCC cell lines,the invasive ability in vitro correlated with uPAR expression;the uPAR （＋） SMMC-7721 possessed higher invasive and mestastasis ability in vitro and in vivo compared with the uPAR（-） ones. Conclusion The expression of uPAR are higher in HCC,and can induce invasion of HCC cells,so uPAR may play significant roles in the development and metastasis of HCC.