摘要
采用分散聚合法合成了粒径可控、单分散的聚苯乙烯微球,考察了单体苯乙烯和引发剂偶氮二异丁腈的浓度以及稳定剂聚乙烯基吡咯烷酮的分子量对微球粒径的影响.采用后修饰法对合成的空白微球进行表面羧基功能性修饰,耦联单克隆抗体的免疫测试结果表明微球表面结合探针分子的能力达到国际同类产品水准.
Microsphere-based assay is a powerful platform to carry out biological detection. The carboxyl functionalized, microsized polystyrene microsphere is important carrier in the microsphere-based assay. Mono-dispersed polystyrene particles with the size range of 2.0 μm to 7.0 μm were synthesized by dispersion polymerization. The surface of microspheres was carboxyl functionalized by post-modification. It is demonstrated that the functionalized polystyrene mierosphere can be well-coupled with monoclonal antibodies with comparable performance achieved as that of commercial carboxyl polystyrene microspheres （Spherotech Inc. ）.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第6期836-839,共4页
Journal of Xiamen University：Natural Science
基金
国家基础科学人才培养基金(J0630429)
2007年度教育部新世纪优秀人才支持计划
教育部留学回国人员科研启动基金
2006年度福建省新世纪优秀人才支持计划资助
关键词
功能性聚苯乙烯微球
液相芯片
微球免疫
functionalized polystyrene particles
suspension array
microsphere-based immunoassay
