鲨鱼皮胶原蛋白肽的成分分析及对血管紧张素转化酶活力的影响

The Effects of Shark Skin Peptides on ACE and the Analysis of Peptides

以鲨鱼皮为原料,采用现代生物化学技术制备胶原蛋白肽水解液,经过喷雾干燥,获得鲨鱼皮胶原蛋白肽干粉.对获得的胶原蛋白肽样品进行成分分析,测定了粗蛋白、粗脂肪、水分、灰分以及K、Na、Ca、Fe、Cu、Zn、Pb等金属元素的含量,应用氨基酸自动分析仪测定样品的氨基酸组成,并用化学分析法测定羟脯氨酸含量为7.08%,应用MALDI-TOF分析胶原蛋白肽分子量的分布,表明我们得到的鲨鱼皮胶原蛋白的水解产物——胶原蛋白肽的分子量主要分布在2～3ku,为水溶性多肽混合物.研究鲨鱼皮胶原蛋白肽对血管紧张素转化酶(ACE)的抑制效应,表明该样品对ACE有较强的抑制作用,其IC50为4.8 mg/mL.本文还探讨了鲨鱼皮胶原蛋白的吸湿性和保湿性.

In this paper, collagen peptide powder from the shark skin was prepared by some biochemistry techniques including proteinase hydrolysis, spray torrefaction. The basic components of the collagen peptide samples were analyzed. The contents of protein, lipid, ash, and the metallic elements such as K, Na,Ca, Fe Cu, Zn, Pb were mensurated. The amino acid composition and content were determined by automatic amino acid analyzer. The content of hydroxyl praline was tested using the chemistry analytical method. The molecular mass ranges of the collagen peptides were determined by the method of MALDI-TOF. The results showed that the collagen peptide powder, we obtained from the shark skin collagen protein by protease hydrolysis, was water-solubility and the molecular mass ranges of most collagen peptides were between 2 ku and 3 ku, The effect of the collagen peptide powder on the activity of angiotensin converting enzyme （ACE） was studied and the results showed that the collagen peptides had inhibitory effect on ACE, the IC50 value was determined to be 4.8 mg/mL. The moisture absorption and maintenance ratio of the collagen peptides was also studied,they were 26.9% and 123% ,respectively.