第三复叶期喷施草甘膦对转基因大豆和普通大豆生理指标的影响

Impact of Glyphosate on Physiological Index of Transgenic and Conventional Soybean on Three-Trifoliolate Leaf Stage

【目的】比较不同大豆品种经草甘膦处理后植株体内生理指标的变化,阐明转基因抗草甘膦大豆与常规大豆对草甘膦及其剂量的生理生化反应差异。【方法】采用随机区组的设计方法,在第三复叶期喷施不同剂量的41%草甘膦异丙胺盐水剂和10%草甘膦水剂,研究其对不同大豆品种叶片叶绿素含量指数、莽草酸含量和SOD活性等生理指标的影响。【结果】41%草甘膦异丙胺盐水剂和10%草甘膦水剂可以抑制抗草甘膦大豆RR1、RR2和普通大豆晋大75、晋豆27叶片的叶绿素合成,抗草甘膦大豆RR1、RR2对两种药剂的抗性高于普通大豆晋大75和晋豆27。4个大豆品种叶片的莽草酸含量随着草甘膦两种制剂剂量的增加而增加,RR1和RR2的增加趋势明显小于晋大75和晋豆27。草甘膦2种制剂对4个大豆品种叶片SOD活性的影响不大。41%草甘膦异丙胺盐水剂对4个大豆品种叶片莽草酸含量的影响大于10%草甘膦水剂,而对叶绿素含量和SOD活性的影响却小于10%草甘膦水剂。【结论】大豆不同品种对草甘膦的敏感程度不同,总的来说抗草甘膦大豆的抗性>普通大豆,RR1的抗性>RR2,晋豆27(晚熟)的抗性>晋大75(早熟)。在这3个生理指标中,敏感性依次为叶片莽草酸含量>叶绿素含量>SOD活性。

[ Obj ective ] An experiment was conducted to study the impact of glyphosate on physiological indexes of different soybean cultivars at three-trifoliolate leaf stage, and to clarify the physiological and chemical differences of glyphosate-resistant soybean cultivars and conventional soybean cultivars on glyphosate. [Method] In the experiment, a random complete block design was used and the impact of spraying 41% glyphosate-isopropylammonium AS and 10% glyphosate AS on physiological index of glyphosate-resistant soybean cultivars （RR1, RR2） and ordinary soybean cultivars （Jinda75, Jindou27） at three-trifoliolate leaf stage was studied. The chlorophyll content index, shikimic acid content and SOD activity were measured. [Result] The results indicated that the chlorophyll content index of four soybean cultivars decreased with increasing the dosage of glyphosate, while sbikimic acid content increased. The impact of 41% glyphosate-isopropylammonium AS on sbikimic acid content was higher than that of 10% glyphosate AS. The impact on chlorophyll content was opposite, and the impact on the SOD activity was little. The impact of 41% glyphosate-isopropylammonium AS on shikimic acid content of the four soybean cu｜tivars was higher than that of 10% glyphosate AS, opposite on chlorophyll content and SOD activity. [Conclusion] There are glyphosate-resistance differences between soybean cultivars. The glyphosate-resistance of glyphosate-resistance soybean is greater than that of conventional soybean. The glyphosate-resistance of RR1 is a little stronger than RR2, and the late-maturing cultivar of conventional soybean is better than early-maturing cultivar. Among the three physiological indexes, shikimic acid content is the most sensitive while SOD activity is the weakest.