[Cr(Ⅲ)(4-ASA)(en)2]Cl配合物的光化学活性

Photochemical Activities of Chromium(Ⅲ) Complex [Cr(Ⅲ)(4-ASA)(en)_2]Cl

通过吸收光谱、荧光光谱、电导率和ESI-MS质谱等方法讨论了铬配合物[Cr(Ⅲ)(4-ASA)(en)2]Cl(4-ASA:4-aminosalicylic acid dianion,en:ethylenediamine)在不同温度、不同pH溶液中的稳定性及光化学稳定性.实验表明,该配合物的溶液(pH7.4)在日光照射下发生了光化学取代反应,取代产物为[Cr(4-ASA)(en)(H2O)2]+.同时研究了配合物及其光照产物对EDTA的动力学反应和对DNA的切割反应.琼脂糖凝胶电泳实验表明,配合物的光化学产物[Cr(4-ASA)(en)(H2O)2]+能有效切割pBR322DNA.

The stability, photochemistry activities and kinetic reaction of chromium complex [Cr（Ⅲ）（4-ASA）- （en）2]Cl·2H2O （4-ASA： 4-aminosalicylic acid dianion, en： ethylenediamine） were discussed by means of UV-visible, fluorescence spectra, conductivity and electrospray ionization mass spectrometry. The complex is sensitive to light. The photochemistry product was deduced to be [Cr（4-ASA）（en）（H2O）2]＋, and then the Gel electrophoresis of plasmid DNA shows that the photochemistry product can promote the cleavage of pBR 322 DNA efficiently.