八种蜂花粉醇提物中总多酚和总黄酮含量测定

The contents of total polyphenols and flavonoids in ethanolic extracts from eight kinds of bee pollens

为测定蜂花粉的功能性成分,用不同浓度乙醇(0%、30%、50%、70%、80%、100%)对杏花、茶花、荷花、油菜、荞麦、向日葵、玫瑰、五味子蜂花粉进行提取。结果表明:八种蜂花粉中多酚和黄酮类物质最适宜用80%乙醇提取,荷花花粉水提多酚提取量高于醇提物。五味子花粉的总多酚含量最高,为19.74±0.20mg/g,荷花花粉的总多酚含量最低,为1.50±0.02mg/g;油菜花粉的总黄酮含量最高,为24.00±0.17mg/g,荷花花粉的总黄酮含量最低,为0.90±0.01mg/g。

To determine functional components of eight kinds of bee pollens, polyphenols and flavonoids were extracted by different concentrations of ethanol, which were apricot pollen, camellia pollen, lotus pollen, rape pollen,buckwheat pollen, sunflower pollen, rose pollen and schisandra pollen. Eight kinds of bee pollens were extracted with different concentrations of ethanol （0%, 30%, 50%, 70%, 80%, 100% ）. The most appropriate extraction concentrations for polyphenols and flavonoids was 80% ethanol. The highest polyphenol content was 19.74±0.20mg/g of schisandra pollen and the lowest was ].50±0.02mg/g of lotus pollen. The highest flavonoid content was 24.00±0.17mg/g of rape pollen and the lowest was 0.90±0.01 mg/g of lotus pollen.