大球盖菇菌丝不同发育期酯酶同工酶研究

Research on esterase isoenzyme of Stropharia rugoso-annulate hypha in growth periods

酯酶同工酶分析是食用菌种质资源研究的生化指标之一,其结果可为大球盖菇的生理生化研究和大球盖菇的应用提供理论和实践参考。用综合PDA液体培养基培养大球盖菇28d,分别于接种后第7、10、13、16、19、22、25、28d用聚丙烯酰胺凝胶圆盘电泳法和醋酸-α-萘酯、醋酸-β-萘酯、固蓝RR盐染色法测定大球盖菇酯酶的活性及种类。结果表明:大球盖菇菌丝生长期先后出现5种酯酶同工酶,Rf值分别是0.48、0.56、0.65、0.73、0.87。5种同工酶的出现时间和活性大小存在差异,第13～16d最多,5种同工酶都有。Rf值为0.56的酶带始终存在,酶活性也较强,可能是大球盖菇菌丝体中酯酶同工酶的特征酶带。

Esterase isoenzyme analysis was one of researched indexes of edible mushroom idioplasm resource. The test results could be for reference in Stropharia rugoso-annulate physiological and biologicalchemistry research and appliance. After Stropharia rugoso-annulate was cultivated 28d in synthetical PDA liquid culture medium,it was inoculated. The activity and kinds of Stropharia rugoso-annulate esterase were determined by polyacrylamide gel electrophoresis and α-Naphthyl acetate,β-Naphthyl acetate and Fast blue RR salt dyeing ways in the 7,10,13,16,19,22,25,28d. The result showed：five kinds of esterase isoenzyme were emerged ,whose Rf were 0.48,0.56,0.65,0.73,0.87. Their emerging periods and activities were not same. They were all in existence from the 13-16α. All the time the enzyme stripe of Rf 0.56 was in enistence and its activity was stronger,which may be characteristic in hypha of Stropharia rugoso-annulate.