凝胶层析分离提纯芦荟多糖的方法

Purifying of Aloe Vera polysaccharide by gel chromatographic separation

研究了采用多孔性凝胶填料为固定相,按分子大小顺序分离样品中各个组分的液相色谱方法。根据此方法使芦荟凝胶汁中的多糖大分子得到进一步分离纯化,同时去除小分子杂质,从而在不改变芦荟生物活性的条件下,有效提高芦荟多糖的含量。

Using the method of HPLC,the multihole gel fillings sample was separated according to the size of molecules as the fixed stationary phase,each component in the On the base of this method, the polysaccharide molecules in Aloe Vera gel juice were separated and purified, and the small molecules of impurities could be moved at the same time, so the polysaccharide content could be improved efficiently under the condition that the Aloe Vera biological activity maintains unchanged.