2种体外肿瘤细胞药敏检测方法的比较研究

Comparative Study of Two Methods of Drug Chemosensitivity Assay in Vitro for Tumor Cells

目的通过WST-8和胶原凝胶微滴肿瘤药物敏感性检测(CD-DST)2种药敏检测方法的比较,对CD-DST法进行评价。方法以胰腺癌细胞系SW1990、PCT-3和ASPC-1为对象,分别采用WST-8和CD-DST法测定其对5-氟尿嘧啶(5-FU)、健择(GEM)和奥沙利铂(OXA)3种药物的敏感性。结果在一定的活细胞数目范围内(500～10000个),CD-DST法所测得的细胞积分光密度与细胞数目成线性正相关(r=0.9911,P<0.05);3种肿瘤细胞的生长抑制率CD-DST法均高于WST-8法(P<0.05),2种方法测定的5-FU、GEM和OXA药物敏感性结果一致。结论CD-DST法可用于胰腺癌的体外药敏检测,具有一定的临床应用价值。

Objective To evaluate the utility of collagen-gel droplet embedded-culture drug sensitivity test （CD-DST） in pancreatic carcinoma cell by compared with WST-8. Methods The chemosensitivity to 5-fluorouracil （5-FU）, gemzar （GEM） and oxaliplatin （OXA） of pancreatic adenocarcinoma cells SW1990, PCT-3 and ASPC-1 were tested by WST-8 and CD-DST respectively. Results In a certain living cell number range （500-10 000）, there was a linear correlation （r=0. 991 1, P〈0, 05） between the integral optical density in CD-DST and the cell number. The inhibition ratios of three kinds of cell growth tested by CD-DST were higher than those tested by WST 8 （P〈0. 05）. The results of drug chemosensitivity to 5-FU, GEM and OXA detected by two methods were u niform. Conclusion The CD-DST can be used to assay the drug chemosensitivity in vitro for pancreatic carcinoma.