多因素量化参数电针对大鼠炎症痛镇痛作用的比较

Comparison of analgesic effects of electroacupuncture of multi-factor quantitative parameters on inflammatory pain in rats

目的:探讨电针治疗大鼠炎症痛最适宜的电针参数方案。方法:将120只Wistar大鼠随机分为12组,即正常空白对照组(空白组)、模型组、10个电针组,电针组分别为A1B1C1、A1B1C2、A1B2C1、A1B2C2、A2B1C1、A2B1C2、A2B2C1、A2B2C2(A12Hz,A2100Hz;B1连续波,B2断续波;C10.1mA,C20.2mA)、A3B3C1(4/20Hz、疏密波、0.1mA)和A3B3C2(4/20Hz、疏密波、0.2mA)。电针组按实验条件分别选取电针参数,每天电针治疗1次,连续6d。通过正交试验设计等方法比较不同频率、波型和电流强度电针对佐剂性关节炎大鼠的镇痛作用,以痛阈和炎症局部组织中β-内啡肽(β-EP)的含量为观察指标。结果:电针治疗提高痛阈的优化参数方案为:100Hz、0.1mA、断续波。电针升高局部组织β-EP含量,100Hz优于2Hz。4/20Hz、疏密波、0.1mA与100Hz、断续波、0.1mA电针对大鼠炎症痛镇痛作用的差异无统计学意义(P>0.05)。结论:电针治疗炎症痛时,最佳参数为100Hz、0.1mA、断续波。

Objective To probe into the best parameter of electroacupuneture （EA） for treatment of inflammatory pain in the rat. Methods One hundred and twenty Wistar rats were randomly divided into 12 groups, normal control group, model group and 10 EA groups including A1B1C1, A1B1C2, A1B2C1, A1B2C2, A2B1C1, A2B1C2, A2B2C2, A2B2C2 （A1：2 Hz, A2： 100 Hz; B1： successive wave; B2： intermittent wave; C1： 0.1 mA, C2： 0.2 mA）, A3B3C1 （4/20 Hz, disperse-dense wave, 0.1 mA） and A3B3C2 （4/20 Hz, disperse-dense wave, 0.2 mA）. The rats of adjuvant-induced arthritis in all of the EA groups were treated by EA with selected different parameters once every day for 6 days. Pain thresholds and β endorphin （β EP）content in the local tissue of inflammation were used as indexes to compare analgesic effects of EA of different frequencies, waveforms and currents by orthogonal experiment design and other methods. Results The optimized parameters of raising the pain threshold was： 100 Hz, 0.1 mA, intermittent wave. EA at 100 Hz was better than 2 Hz for increasing the content of 13 EP in local tissue of inflammation. The analgesic effect of EA at 4/20 Hz, 0.1 mA, disperse-dense wave on the inflammatory pain in the rat was not significant different with that at 100 Hz, 0.1 mA, intermittent wave （P〉0.05）. Conclusion The best parameters are 100 Hz, 0.1 mA and intermittent wave for EA treatment of inflammatory pain in the rat.