摘要
将人工杂交选育的坛紫菜新品系Z-26,Z-61,Z-81,Z-17的子3代(F3)与野生型对照组进行遗传性状的比较,旨在筛选耐高温品系。实验结果表明:(1)在高温29℃下培养40 d,人工选育的4个新品系丝状体均能正常生长发育,其中Z-26丝状体生长较快,Z-61在29℃下较易成熟,95%的营养藻丝发育成孢子囊枝,Z-17只有30%形成孢子囊枝;(2)Z-26和Z-61品系的叶状体在30℃下培养10 d,虽然藻体变白,但没有死亡,此时将其移至21℃下恢复培养3-4 d,藻体逐渐恢复为原有的色泽;(3)Z-81和Z-17的叶状体在29℃下培养7-10 d,虽然藻体发白但尚未死亡,移至21℃下培养基本可恢复正常,但在30℃下培养10 d,藻体腐烂不可恢复;(4)野生型对照组不耐高温,藻体在27℃下培养5 d就腐烂死亡,人工选育的4个品系耐受高温的幅度比野生型对照组高1-3℃;(5)4个品系叶状体在26-28℃温度下培养10 d后,其主要的藻胆蛋白和叶绿素a的含量与21℃培养下的含量差异不显著,说明短时间的高温培养并不影响其品质。
Owing to selecting the strains of high temperature resistance, F3 generation of new strains coming from hybridization in Porphyra haitanensis (Z-26, Z-61, Z-81, Z-17) was compared with the wild-type control group by inheritable traits. Results indicated that: (1) The four new strains of selective filament strains could normally grow and develop after 40 d cultivated at 29 ℃ high temperature. Such as Z -26 filament grew faster; Z-61 was easily mature and 95% of the conchocelis filament sprouted into sporangial branch. There was only 30% of filament sprouted into sporangial branch in Z-17. (2) Z-26 and Z-61 were cultivated 10 d under the temperature of 30 ℃. Then they turned white but were not dead and could return their intrinsical colour after 3-4 d cultivated at 21 ℃. (3)Blades of Z-81 and Z-17 which were cultivated 7-10 d at 29 ℃ turned white but were not dead and could return normal at 21 ℃. However, when cultivated 10 d under the temperature of 30 ℃, they were rotten and could not be recovered. (4) Wild-type control group was no high temperature resistance and they were rotten even dead after 5 days cultivated under the temperature of 27 ℃. The four stains of slection could resist the high temperature, 1-3 ℃ higher, comparing with the wild-type control group. (5) The thallus of the four strains were cultivaried 10 d under the temperature of 26-28 ℃ and their main phycobiliprotein and chlorophyll a were quiet variance comparing with the thallus that were cultivated under the temperature of 21 ℃. It indicated that temporary high temperature did not make effect on their quality.
出处
《海洋学报》
CAS
CSCD
北大核心
2008年第5期100-106,共7页
基金
国家海洋"863"资助项目(2006AA10A413)
国家自然科学基金资助项目(40676077)
国家科技支撑项目(2007BAD07B03)
关键词
坛紫菜
品系
高温
生长发育
Porphyra haitanensis
strains
high temperature
growth and development