与黄瓜矮生基因连锁的ISSR标记及其SCAR转换

Molecular Tagging of ISSR and Conversion of SCAR Marker Linked to a Mutant Dwarf Gene in Cucumber

以黄瓜矮生品系D8与蔓生品系JIN5杂交并自交得到的124株F2代为试材,应用ISSR分子标记技术和BSA(混合群体分组分析)法寻找与黄瓜矮生基因连锁的分子标记。从80条ISSR引物中筛选到一个黄瓜矮生性状特异的多态性引物UBC818。经F2代单株验证,UBC818的扩增片段在蔓生黄瓜JIN5植株中稳定出现,而在矮生黄瓜D8植株中没有。连锁关系分析表明,标记与黄瓜矮生基因间的遗传距离为11.1cM。根据对该片段的序列分析结果重新设计了1对引物,将ISSR标记成功转化成了SCAR标记,并命名为UBC818-S。

In this study, 124 of F2 plants from the cross combination of the dwarf cucumber D8 and the vine plant JIN5 were used to screen the molecular marker linked to the dwarf mutant gene in cucumber with ISSR molecular tagging and BSA （bulked segregation analysis） method. UBC818, a specific primer of polymorphism, was obtained from eighty ISSR primers screening for a cucumber dwarf traits specific in the dwarf line. The marker from the results of PCR with UBC818 was tested in individual DNAs reaction for the F2 population and the UBC818 amplified fragment could only be detected in the vine plants. Linkage analysis showed that the genetic distance between the specific marker and the cucumber dwarf mutant gene was 11.1 cM. According to the sequence of the fragment analysis, one pair of primers had been designed. The ISSR marker was successfully converted into a SCAR marker which was named UBC818-S.