利用微卫星DNA标记对南海湛江海域军曹鱼群体遗传多样性的分析

A genetic analysis of population of cobia,Rachycentron canadum around Zhanjiang waters of South China Sea with microsatellite markers

从10对美国密西西比州海泉市近海军曹鱼Rachycentron canadum的微卫星引物中选出5对多态性好的引物对中国南海湛江海域军曹鱼48个个体进行遗传多样性研究。结果表明,5个微卫星位点的等位基因数目、有效等位基因数、多态信息含量和杂合度分别在2—9、1.552 9—7.339 7、0.324 2—0.856 3和0.356 0—0.863 8之间,说明中国南海湛江海域军曹鱼的遗传多样性处于较高的水平。通过比较,证明其遗传多样性比密西西比州海泉市近海军曹鱼的遗传多样性丰富。建议在军曹鱼资源没有受到破坏时,采取相应的策略对其进行保护。

Cobia, Rachycentron canadura, is an economically important fish which mainly distributes in tropical warm waters worldwide. In order to collect enough data to study the status of germplasm re sources of this fish, 10 pairs of microsatellite markers of the R. canadum population from the Mississippi of the southeast United States were selected to analyze the genetic diversity of the R. canadum population around Zhanjiang waters of the South China Sea. Five pairs of the markers were proved useful and assessed the genetic diversity of 48 individuals of R. canadum. The results show that the numbers of alleles and effective alleles and the values of polymorphism information content and heterozygosity ranged from 2-9, 1. 552 9-7. 339 7, 0. 324 2-0. 856 3, and 0. 356 0-0. 863 8, respectively, indicating that the diversity of the R. canadum population around Zhanjiang waters was relatively high, even higher than that of the R. canadum population in the Mississippi by comparison. It is suggested that adequate measures should be taken to protect R. canadum resources in China before it is disturbed by human activities.