EGFR和LRP mRNA在卵巢上皮性癌中的表达及意义

The Expression of Epidermal Growth Factor Receptor and Lung Resistance Protein mRNA in Primary Epithelial Ovarian Cancer and Its Clinical Significance

目的探讨表皮生长因子受体(epidermal growth factor receptor,EGFR)和肺耐药蛋白(lung resistanceprotein,LRP)mRNA在卵巢癌中的表达及相互关系。方法用逆转录聚合酶链反应(RT-PCR)技术检测12例正常卵巢、11例卵巢良性肿瘤及35例卵巢上皮癌组织中EGFR mRNA和LRP mRNA的表达情况,并分析它们之间的相关性。结果卵巢上皮癌组织中EGFR mRNA阳性表达率显著高于正常卵巢及卵巢良性肿瘤组织(P<0.05);EGFR mRNA表达与卵巢上皮癌手术病理分期有关,Ⅲ-Ⅳ期的阳性表达率及表达强度高于Ⅰ-Ⅱ期(P<0.05);LRP mRNA表达与卵巢上皮癌患者年龄、临床分期、分化程度、病理类型及淋巴结转移等临床病理参数均无关(P>0.05);EGFR mRNA和LRP mRNA的表达水平与化疗敏感性有关;EGFR和LRP基因表达之间有显著相关性(r=0.729,P<0.01)。结论EGFR和LRP基因高表达与卵巢癌化疗耐药的产生有一定相关性,二者可能有协同作用。检测卵巢癌EGFR和LRP对靶向化学治疗可能有指导作用。

Objective To investigate the interrelationship between epidermal growth factor receptor （EGFR） mRNA and lung resistance protein （LRP） mRNA in ovarian carcinoma. Methods Reverse transcription polymerase chain reaction （RT-PCR） was employed to determine the expression of EGFR mRNA and LRP mRNA in 58 ovarian specimens,including 12 normal, 11 benign and 35 malignant,and their relationship was analyzed. Results The positive rates of EGFR mRNA and LRP mRNA were significantly higher in malignant ovarian specimens than those in the normal and benign ones （P〈0.05）. There was correlation between EGFR mRNA expression and clinical stage and stage Ⅲ - Ⅳ showed a higher positive expression rate than stage Ⅰ - Ⅱ （P〈0.05）. The expression of LRP mRNA was not associated with age, clinical stage, differentiation,histological type and lymph node metastasis in epithelial ovarian cancer （P〉0. 05）. Increased EGFR mRNA and LRP mRNA level had correlation with lower sensitivity to chemotherapy. There was correlation between EGFR and LRP（r = 0. 729, P%0.01）. Conclusion The expressions of EGFR and LRP might be related to ovarian carcinoma multidrug resistance.