p27基因在宫颈癌中的表达及其临床意义

Expression of p27 in Human Cervix Carcinoma and Its Clinical Significa Nce

目的探讨p27基因在子宫颈癌中的表达及临床意义。方法采用免疫组织化学方法,分别检测30例正常宫颈组织、32例宫颈非典型增生(CINⅢ)和70例宫颈癌(包括30例腺癌、40例鳞癌)组织中的p27的表达情况。结果p27蛋白在正常宫颈、宫颈非典型增生、宫颈癌组织中的高表达率分别为96.67%、62.50%、40.00%,两两比较,差异有统计学意义(P<0.05)。p27蛋白的表达与组织学分级、淋巴结转移与否有关(P<0.05);p27表达阳性的患者其预后优于p27表达阴性的患者,两者比较差异有统计学意义(P<0.05)。结论p27与宫颈癌的发生、分化程度、淋巴结转移及预后有关;对p27蛋白进行检测可为诊断宫颈癌、估计预后及临床综合治疗提供参考。

Objective To investigate the clinicopathological significance of p27 gene expression in cervical carcinomas. Methods Using immunohistochemistry technique, we examined the expression of p27 protein in 30 cases of normal cervix uteri, 32 of cervical intraepithelial neoplasia, and 70 of cervix carcinoma （30 cases cervical adenocarcinoma and 40 cases squamous cervical carcinoma）. Results The high expression rate of p27 in normal cervix uteri, cervical intraepithelial neoplasia and cervical adenocarcinoma were 96. 67 %, 62. 50%, 40. 00%. Compared with each other, they had a high significant difference（P〈0.05）. The expression of p27 in cervical carcino- ma was relate to histologic grade and lymph node metastasis （P〈0. 05） and the prognosis of the positive expression of p27 protein group was better than that of the negative expression of p27 protein group; statistically there was difference between the two groups （P〈0.05）. Conclusion p27 is related to the occurrence, differentiation degree and lymph node metastasis and prognosis of cervical carcinoma. Detection of p27 protein expression could provide referring criteria for its diagnosis, predictor of prognosis and clinical comprehensive therapy.