缺氧缺血(HI)新生大鼠脑损伤后NO水平的变化及GM1对其影响

Effects of GM1 on the Level of NO after Hypoxic-ischemic Brain Damage in Neonatal Rats

目的:观察新生大鼠缺氧缺血性(HI)脑损伤后NO水平的变化,探讨单唾液酸四己糖神经节苷脂(GM1)对NO水平的影响。方法:选择新生7日龄Sprague-Dawley大鼠108只,随机分为三组:缺氧缺血组(HI组,36只),假手术对照组(Control组,36只),缺氧缺血+神经节苷脂组(GM1组,36只)。采用结扎右侧颈总动脉并吸入低氧混合气体制备HIBD模型。采用镀铜镉还原法测定血浆一氧化氮(NO)水平。结果:Control组、HI组、和GM1组新生大鼠血浆NO-2/NO-3含量分别为(41.6±8.9)、(60.9±14.7)和(43.8±10.6)μmol/L。HI组明显高于对照组和GM1组(P<0.05);GM1组与对照组比较,无明显差异(P>0.05)。结论:GM1能抑制新生大鼠缺氧缺血性(HI)脑损伤后NO生成,对缺氧缺血(HI)新生大鼠的脑具有保护作用。

Objective： To observe the changes in the level of NO after hypoxie - ischemie brain damage in neonatal rats and to disscuss the effect of GMI on the level of NO. Methods： 108 cases of 7 - day - old Sprague - Dawley rat were divided at random into 3 teams ： hypoxic - ischemic - team （ HI team,36 cases）, false -oprational control team（ control team 36 cases） , hypoxic - ischemic - GM1 （ GM1 team,36 cases）. Deligation of cephalic artery and breathing in low -oxygen gas mixture was applied to prepare HIBD mode. the NO content in blood plasma was assayed by plated copper cadmium reduction. Results：The content of NO2 -/NO3 - in neonate rat blood plasma of Control team,HI team,and GM1 team was according to （41. 6 ± 8.9 ）, { 60.9 ± 14.7 ） and （ 43.8±10.6 ） μmol/L. it is obviously higher in HI team than both Control team and GMlteam （ P 〈0.05 ）. There are no significant difference between Control team and GMI team（P 〉0.05）. Conclusion： GMI can inhibit the production of NO and cell apoptosis in CAI Region of the Hippocampus after hypoxic -ischemic brain damage in neonatal rats. It is a protection to the brain of hypoxic -ischemie neonatal rats.