中国人群FⅫ 46C/T多态性与血栓形成关联性解析

Research on relativity between FⅫ 46C/T Polymorphism and thrombosis in the Chinese

目的探讨FⅫ基因exon146C/T基因多态性与血栓形成的关联性。方法采用PCR-RFLP法鉴定了92例血栓性脑梗塞患者和87例静脉血栓患者及129例健康人的FⅫ基因exon146C/T基因型及C、T等位基因分布频率,采用多元Logistic非条件回归分析方法,进行多因素分析,解析FⅫ基因46C/T多态性与血栓性脑梗塞、静脉血栓形成的关联性。结果血栓性脑梗塞组和静脉血栓组的C等位基因分布频率均高于对照组,血栓性脑梗塞组的C/C基因型在血栓形成中的危险性是T/T基因型的14.533倍,具有统计学意义;静脉血栓组中C/C基因型P值为0.074(χ2=3.203,OR=2.063,95%CI:0.933-4.559),没有统计学意义。结论中国人群中存在FⅫ46C/T基因多态性位点。FⅫ46C/C基因型可能是脑血栓形成的危险因素,而静脉血栓的形成可能与FⅫ46C/T基因多态性没有关联性。

Objective To explore the association between the 46C/T gene polymorphism in the exon-1 region of the coagulation factorⅫ （FⅫ） and thrombosis. Methods PCR-RFIP method was used to investigate the distribution frequency of the 46C/T gene polymorphism and C or T allele among 92 thrombetic eerbral infarction patients, 87 venous thrombosis patients and 129 healthy controis. The relation of the 46C/T gene polymorphism with thrombosis and thrombotie cerbral infarction was analyzed by Logistic an - conditional regression. Results The distribution frequeney of C allele in both thrombotic eerbral infarction patients and venous thrombosis patients was higher titan healthy controls. The risk of C/C genetype of thrombotic cerbral infarction patients in the thrombosis was 14. 533 times higher than T/T genetype of that and there was significant difference. The P value of C/C genetype in the venous thrombosis patients was 0.074 （χ^2 = 3.203, OR = 2. 063,95 % CI： 0.933 - 4.559. There was no significant difference. Conclusion There was FⅫ exonl 46C/T gene polymorphism site in rite Chinese. The risk factor of cerebral thrombosis was FⅫ exonl 46C/C gene polymorphism. There was no relafivity between the deep venous thrombosis and 46C/T gene polymorplfism.