纳米羟基磷灰石／丝素蛋白生物复合材料的制备和表征

Preparation and Characterization of Nano-Hydroxyapatite/Silk Fibroin Biocomposites

采用硝酸钙-丝素蛋白溶液与磷酸钠原位合成纳米羟基磷灰石(HA),在反应过程中丝蛋白(SF)诱导HA晶体生长,仿生合成HA/SF复合材料,用TEM、IR、TGA、XRD和SEM进行表征。结果表明,HA为CO23-部分替代型,粒径为10 nm^40 nm之间的弱结晶类骨晶体,在形貌和结晶度等方面与人体骨磷灰石相似。HA/SF复合材料中丝蛋白的含量约为25%,HA和SF两相存在强烈化学键合作用。SEM观察结果表明,HA微粒被SF完全包裹,两者之间没有明显相分离。

HA/SF Biocomposites were prepared by coprecipitation method from SF-Ca（NO3）2 and Na3PO4 solution, in the process of the reaction Silk fibroin acted as template to induce the directional growth of HA crystals. The mechanism of biomimetie synthesis of the HA was discussed and the constitute, structure and morphology of HA/SF composites were studied by TEM, IR, TGA, XRD and SEM. The results indicate that the needle-like HA crystals are poorly-crystallied and partially substituted by CO3^2- and similar to the crystals of human bone in morphology, structure, components and size, the diameter is 10 nm- 40 nm. The content of SF in the composite is about 25 %, and the two phases bonded each other strongly. SEM results show that the HA particles are enwraped entirely with SF and no obvious separation between the two phases of the composite was observed.