血管内皮生长因子、血管生成素-2表达及微血管密度与肺癌预后的关系

Effects of the Expressions of VEGF and Ang-2 and MVD on the Prognosis of Lung Cancer

目的研究肺癌中血管内皮生长因子(vascular endothelial growth factor,VEGF)、血管生成素-2(an-giopoietin-2,Ang-2)表达水平与微血管密度(Microvascular density,MVD)的相关性,探讨三者对肺癌生存率的影响及与生存期的相关性,为判断肺癌预后提供依据。方法免疫组化法检测96例肺癌手术切除标本中VEGF、MVD表达,western-blotting检测肺癌标本中Ang-2表达变化。结果肺癌标本中VEGF、Ang-2高表达,MVD高表达组患者5年累积生存率分别为17.2%、16.3%、15.5%,显著低于低表达组(37.5%、31.9%、36.8%)(P<0.01);VEGF、Ang-2基因表达,MVD与术后生存期负相关(P<0.05);VEGF、Ang-2基因表达水平与MVD正相关(P<0.05)。结论VEGF、Ang-2表达水平越高,MVD越大,肺癌患者生存率越低,生存期越短,是判断肺癌预后有价值的指标。抑制VEGF、Ang-2的表达来降低MVD可能成为治疗肺癌的新靶点。

Objective To explore the relationship between VEGF, Ang-2 gene expression and MVD,and their effects on the prognosis of lung cancer. Methods The expression of VEGF and MVD of 96 lung cancer tissues were detected by immunohistochemical staining （LSAB Methods）, and of Ang-2 of 96 lung cancer tissues were detected by western - blotting. Results The 5 - year cumulative survival rates of groups with high expression of VEGF and Ang - 2 and high MVD were 17.2%, 16.3% and 15.5%, which were significantly lower than that of groups with low VEGF, Ang -2 expression and low MVD（37.5%, 31.9% and 36.8% respectively） （P 〈 0.01 ）. The survival rate of lung cancer patients was negatively related to the expression of VEGF, Ang-2 and MVD （P〈0.05 ）. The expression of VEGF and Ang-2 in lung cancer tissue was positively related to MVD. Conclusion The higher expression of VEGF and Ang- 2 and higher MVD the tissue presented, the lower survival rate and lower survival duration occurred, so the expression of VEGF and Ang -2 and MVD of tissue can be used to determine the prognosis of lung cancer, and inhibition of the expression of VEGF and Ang -2 and MVD maybe a way of curing lung cancer.