固相萃取-反相高效液相色谱法测定小麦中的嘌呤类物质

Determination of Purines in Wheat by Solid Phase Extraction-Reverse Phase High Performance Liquid Chromatography

采用Sep-Pak C18萃取柱,建立了固相萃取-反相高效液相色谱法测定啤酒酿造辅料小麦中的嘌呤类物质含量的方法。该方法准确可靠,重现性好,4种嘌呤的回收率为90.6%～96.4%,相对标准偏差（RSD）小于3%。腺嘌呤（Ade）、鸟嘌呤（Gua）、黄嘌呤（Xan）和次黄嘌呤（Hyp）的最低检测限依次为0.1,0.2,0.1,0.5 mg/L。

An analytical method of purines in beer was established by RP-HPLC. The recoveries of purines were between 90.6-96.4%, and the relative standard deviation was below 3%. The limit of detection for ademine, guamine, xanthine and hypoxanthine were 0.1 mg/L, 0.2 rag/L, 0. 1mg/L and 0.5 mg/L, respectively. It was proved to be a convenient and accurate method for analyzing purines content in wheat and wheat malts, and it provided a reliable method for the research of beer with low purines.