深圳市人民医院沙门菌对抗菌药物敏感性分析

Antimicrobial resistance in the Salmonella strains isolated from Shenzhen People's Hospital

目的监测我院2002—2007年临床分离沙门菌对各类抗菌药物敏感性,指导临床合理使用抗菌药物。方法采用琼脂稀释法检测各类抗菌药物对95株沙门菌MIC,数据分析采用WHONET5.3软件。结果伤寒沙门菌和甲型副伤寒沙门菌对氨苄西林、氯霉素、复方磺胺甲口恶唑敏感率仍分别高达96%～100%和97%～100%,未发现多重耐药株(MDR);52%(13/25)伤寒沙门菌和94%(62/66)甲型副伤寒沙门菌对萘啶酸耐药,对环丙沙星敏感性降低(MIC=0.125～1mg/L)伤寒和甲型副伤寒沙门菌分别占24.0%(6/25)和92.4%(61/66);1株乙型副伤寒沙门菌和1株鼠伤寒沙门菌对环丙沙星等氟喹诺酮类药物高耐药(MIC≥16mg/L),且对氨苄西林、氯霉素和复方磺胺甲口恶唑多重耐药。结论我院环丙沙星敏感性降低伤寒和甲型副伤寒沙门菌较常见,出现高耐氟喹诺酮类药物且多重耐药的乙型副伤寒沙门菌和鼠伤寒沙门菌,应加强对沙门菌耐药性监测。

Objective To investigate the antimicrobial resistance among the Salmonella strains isolated from Shenzhen People＇s Hospital. Methods The minimal inhibitory concentrations （MICs） of antibacterial agents were determined by agar dilution meth od against 95 strains of Salmonella. WHONET 5.3 software was used to analyze the data. Results S. typhi and S. paratyphi A remained highly susceptible to ampicillin, chloramphenicol and trimethoprim-sulfamethoxazole. About 96%-100% and 97%-100% of the strains were susceptible respectively. Multidrug-resistant isolate was not found. About 52% （13/25） of S. typhi isolates and 94% （62/66） of S. paratyphi A isolates were resistant to nalidixie acid, respectively. The prevalence of S. typhi and S. paratyphi A with decreased susceptibility to ciprofloxacin （MIC = 0. 125 -1 mg/L） was 24% （6/25） and 92.4% （61/ 66）, respectively. One strain of S. paratyphi B and one strain of S. typhimurium showed high resistance to ciprofloxacin （MIC≥16 mg/L）, and resistance to ampicillin, chloramphenicol and trimethoprim-sulfamethoxazole too. Conclusions The S. typhi and S. paratyphi A with decreased susceptibility to ciprofloxacin were prevalent in our hospital. Ciprofloxacin-resistant S. paratyphi B and S. typhimurium were identified.