survivin mRNA在胆管癌病人血清中的表达和意义

Expression of survivin mRNA in serum of patients with cholangiocarcinoma

目的检测胆管癌病人血清中survivin mRNA的表达,探究其临床意义。方法应用RT-PCR检测20例胆管癌患者、41例良性胆道疾病患者、10例健康志愿者血清中survivin mRNA的表达,并与胆管癌的病理分级进行相关性分析。结果在20例胆管癌病人、41例良性胆道疾病病人、10例健康人血清中,survivin mRNA的阳性表达率分别为85.0%(17/20)、21.9%(9/41)、0(0/10),前者血清中survivin mRNA的阳性表达水平明显高于后两者血清中的阳性表达水平(P<0.05)。对胆管癌病人的病理分级进行相关性分析显示,其血清中survivin mRNA的表达与病理分级无相关性。结论在绝大多数胆管癌病人血清中都表达survivin mRNA,可以做为诊断胆管癌的一种新型肿瘤标记物。

Objective To explore the expression of survivin mRNA in serum of patients with cholangiocarcinoma and its significance. Methods The expression of survivin mRNA was detected by RT-PCR in serum of 20 patients with eholangiocarcinoma,41 patients with benign biliary tract disease and 10 healthy controls. The correlation between the expression of survivin mRNA and clinicopathologieal parameters in cholangioncarcinoma was analyzed. Results Survivin mRNA was detected in 17 patients（79.2 % ） with cholangiocarcinoma,9 patients（21.9% ）with benign biliary tract diseases, but not in healthy controls. The expression level of survivin mRNA in serum of cholangiocarcinoma patients was significantly higher than that in patients with benign biliary tract disease and healthy controls（ P 〈 0.05）. No correlation was found between the expression of survivin rnRNA and tumor staging. Conclusion Survivin mRNA may be a novel tumor marker for diagnosis of cholangiocarcinoma.