结肠黑变病相关基因的cDNA芯片筛查分析

Study on differentially expressed genes in melanosis coli by cDNA microarray

目的应用基因芯片技术研究结肠黑变病(MC)和正常成人结肠组织基因的差异表达。方法分别用Cy5和Cy3两种不同的荧光染料通过逆转录反应将MC组和对照组结肠组织的mRNA分别标记成探针,并与载有一组靶基因的基因表达谱芯片进行杂交。通过扫描荧光强度,计算机软件分析,寻找两组差异表达基因。结果MC组和正常对照组之间共筛选出93条差异表达基因,其中表达增加的基因有53条(2.0倍以上),表达降低的基因有40条(0.5倍以下)。结论基因表达谱芯片筛选MC与正常成人结肠组织差异表达基因具有样品用量少、高速度、高敏感、高通量等特性。通过筛选所得差异基因提示MC的发病可能涉及细胞增殖、酯类代谢、能量代谢、细胞毒等多种因素,为进一步阐明MC的发病机制提供了新线索。

Objective To explore the differences of the differentially expressed genes in colon tissues of patients with Melanosis coli （MC）and normal controls by cDNA microarray. Methods The mRNA from MC and normal colon tissues were reversely transcribed to cDNA with incorporation of fluorescent dCTP（Cy5 or Cy3）to prepare for hybridization probes. The mixed probes were hybridized with the cDNA microarray. The differential genes in 2 groups were detected by scanning the fluorescent signals. Results All together, 93 differentially expressed genes were found in MC and normal colon tissues, of which, 53 genes showed increased expression and 40 genes showed decreased expression. Conclusion The result concludes that cDNA microarray technique is a suitable method in screening the differentially expressed genes in tissues, and can be used for understanding pathogenesis in melanosis coll.