TaqMan探针荧光定量PCR检测甜菜低温胁迫相关miRNAs

TaqMan qRT-PCR assay for quantitative identification homologous miRNAs in Beet (Beta vulgaris) responsible to cold stress

本项研究根据模式植物拟南芥中miR156,157,159,167,319,396在植物中所具有的广泛同源性及其在非生物胁迫环境下可能具有的共同调控机制,采用TaqMan探针实时荧光定量PCR技术检测低温胁迫下甜菜幼苗同源miRNAs的差异表达,以期揭示甜菜响应低温胁迫的可能分子机制。实验表明,甜菜中存在与拟南芥同源的miR156,157,159,167,319,396基因,miR159基因表达稳定,在本实验中用作定量内参,miR156,157,167,396明显下调,而miR319虽然被认为与miR159属于同一家族,但在甜菜幼苗低温胁迫下表现为明显上调,说明miR156,157,167,319,396是甜菜中响应低温信号的调控分子。本研究首次识别甜菜中存在与拟南芥同源的6个miRNAs,为进一步探索甜菜响应低温胁迫的分子机理奠定了技术。

microRNAs （miRNAs） is a kind of non- coding RNAs which regulates post- transcriptional expression in eukaryotie cells. The TaqMan qRT - PCR developed by ABI has been validated as one of the most promising methods. Although beet is one of the most important materials, its miRNAs identification has not reported. This article reported 5 homologous miRNAs in beet （ Beta vulgaris ） responsible for cold stress with TaqMan qRT- PCR.