新疆天然雪莲与其组织培养物中水溶性多糖的研究

Study on the Water Soluble Polysaccharide of Sausurea involucrate and Cultivated Sausurea involucrate

目的对新疆天然雪莲及其组织培养物进行多糖提取分离纯化,为合理利用雪莲提供一定依据。方法雪莲经热水浸提,乙醇沉淀得粗多糖。粗多糖经Sevage法和酶法联合脱蛋白,得初步纯化的新疆天然雪莲多糖(XJXL1)和培养雪莲多糖(PYXL1)。结果纸层析和气相色谱分析表明XJXL1和PYXL1是由多种单糖组成的酸性杂多糖。XJXL1和PYXL1对超氧阴离子自由基和羟自由基具有明显的清除效果,且对细菌有很好的抑制作用。结论从多糖含量、清除自由基及抑菌活性实验可以证明新疆雪莲培养物是新疆天然雪莲的良好替代物,具有进一步开发生产的价值。

Objective To provide scientific cue for the use of Sausurea involucrate, Xinjiang Sausurea involucrate and tissue cultures were isolated and purified for polysacchafide. Methods The crude polysaccharide was extracted from Sausurea involucrate with hot water ,and precipitated by ethanol. The refined polysaccharide was prepared after eliminating protein. Results By using PC analysis and GC analysis, it was found that polysacchafide of Sausurea involucrate was composed of different kinds of sugar. Polysaccharide of Sausurea involucrate was effective in scavenging superoxide radical and hydroxyl radical. Furthermore, they had considerable antibiotic activity against bacteria, but had no inhibitory effects on epiphyte. Conclusion Cultivated Sausurea involucrate is a good substitute for crude Saussurea involucrate from the rate of extracted polysaccharide content, polysacchafide content, scavenging free radicals and antibacterial activity of the experiment.