岩黄连生物总碱对肝纤维化大鼠TGF-β1及MMP-9的影响

Protective Effect of Corydalis saxicola Alkaloids(CSA) on Level of TGF-β1,MMP-9 in Rats with Liver Fibrosis

目的观察岩黄连生物总碱对肝纤维化大鼠TGF-β1及MMP-9表达的影响,并探讨其抗纤维化的可能机制。方法Wistar大鼠80只随机分为6组,除正常组外其他各组首次皮下注射50%CCl45 ml.kg-1,以后每3天注射1次30%CCl43 ml.kg-1。正常组给予等体积花生油,持续12周,给药自造模之日开始。结果CCl4所致慢性肝纤维化大鼠有明显的肝损伤及慢性肝纤维化的表现。与模型组相比,岩黄连生物总碱三个剂量组能不同程度改善肝功能指标;肝组织中Hyp含量较模型组明显降低;病理组织学检查表明其肝脏病变程度较模型组明显减轻;肝组织中TGF-β1,MMP-9的表达较模型组明显下降。结论岩黄连生物总碱对慢性肝纤维化大鼠具有肝保护作用及抗肝纤维化作用,可能通过抑制TGF-β1和MMP-9表达而促进肝纤维化的逆转。

Objective To observe the effects of Corydalis saxicola alkaloids （CSA） on the levels of TGF -β1, MMP- 9 in the livers of rats with hepatic fibrosis. Methods Hepatic fibrosis was induced by hypodemic injection of 40% CCl4 （5ml · kg^-1 for the first time,3 ml · kg^-1 every 3 days）. Results Obvious liver damage and chronic fibrosis were observed in the rats after the injection of CCl4. The parameters reflecting liver function were improved in the three groups administered with various dose of CSA ; the hydroxyproline in the liver was obviously declined; the histopathological improvement in the liver damaged by CCl4 was achieved in CSA - treated groups ; levels of TGF -β1 and MMP - 9 in the CSA - treated groups were significantly lower than those in the liver fibrosis group. Conclusion CSA is efficient for the treatment of hepatic fibrosis. The mechanisms possibly involve the inhibition of TGF - β1 and MMP -9.