重型颅脑损伤后TNF-α表达及其与细胞凋亡的关系

The study on the expression of TNF-α after traumatic brain injury and the relationship between it and apoptosis

目的探讨肿瘤坏死因子-α(TNF-α)在大鼠重型颅脑损伤后各个阶段的表达水平及其与颅脑损伤后细胞凋亡的关系。方法将36只大鼠随机分为实验组与对照组。实验组大鼠制作重型颅脑损伤模型,对照组大鼠予以假手术处理,两组大鼠分别在伤后6、24与72h通过RT-PCR法检测TNF-αmRNA水平、TUNEL法检测细胞凋亡水平。结果重型颅脑损伤后大鼠脑组织TNF-αmRNA水平、细胞凋亡水平均较对照组明显升高,并在伤后24h达到高峰。结论TNF-α可能在颅脑损伤后的细胞凋亡中起了重要的作用,对TNF-α表达的干预可望起到减少神经元凋亡从而保留更多神经功能的目的。

Objective To analyze the expression of TNF- α after severe traumatic brain injury （TBI） in SD rat, as well as the relation between the expression and apoptosis of cell after TBI. Methods Thirty-six SD rats were divided to study group and control group randomly. In study group, the animals were executed at the time of 6h, 24h, and 72h after employing model of severe TBI. And thus the expression of TNF- α mRNA was evaluated through RT-PCR, and apoptosis of cell was evaluated through TUNEL. In control group, the expression of TNF-α mRNA and apoptosis were evaluated through the same way but without employing model of severe TBI. Results After severe TBI, the expression of TNF- α mRNA and apoptosis increased, and the peak showed up 24h after TBI. Conclusion TNF- α probably takes effect on the apoptosis after TBI, and the intervention of expression TNF- α may lead to the less apoptosis.