异源新城疫病毒感染对雏鸭组织抗氧化酶活性的影响

Effect of ducks infected with heterologous Newcastle disease virus on tissue free radicals metabolisms

选择隔离饲养至20日龄的健康雏鸭120只,随机分为4组,Ⅰ组为对照组,皮下注射0.5mL/只灭菌生理盐水,Ⅱ、Ⅲ、Ⅳ组分别皮下注射0.5mL/只1∶5稀释的鸡源、鹅源和鸭源新城疫病毒SPF胚液,并分别置于25℃隔离环境下饲养与观察。分别于感染后4、8、12、16d每组随机抽取雏鸭7只颈静脉放血致死,采集心、肝、脾、肺及肾组织,制成10%的组织匀浆液,测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)的活性或含量,探讨异源新城疫病毒感染对雏鸭组织自由基代谢的影响。结果表明,感染异源新城疫病毒的雏鸭组织SOD、CAT、GSH-Px活性和MDA含量变化不同,感染鸭源和鹅源新城疫病毒的雏鸭组织抗氧化酶的活性变化明显,而感染鸡源新城疫病毒的则变化不明显。这说明不同来源的新城疫病毒对雏鸭的感染性有一定差异。

One hundred and twenty 20 day-old healthy ducks were divided randomly into 4 groups（ Ⅰ,Ⅱ,Ⅲ,Ⅳ ） and subcutaneously injected with 0.5 mL sodium chloride,SPF embryo solution（diluted by 1 ： 5） of Newcastle disease virus separated from chicken, goose and duck, respectively. Ducks were raised and observed in housing with 2,5 ℃. Seven ducks in every group were slaughtered 4,8,12,16 d post-infection. The heart, liver, spleen, lung, kidney were sampled and grinded into 10% homogenate. Superoxide dismutase （ SOD）, catalase （ CAT）, malondialdehyde （MDA） ,glutathione superoxidase （GSH Px） were measured to study the effect on tissue free radicals metabolisms of ducks infected with heterologous Newcastle disease virus. The results showed that the content of SOD, CAT, MDA,GSH Px in duck tissues changed with the beterologous Newcastle disease virus infection. There were obvious difference of the content of SOD, CAT, MDA, GSH-Px in duck tissues infected with Newcastle disease virus from goose, Newcastle disease virus from duck, but no significantly difference of the content of SOD,CAT, MDA,GSH-Px in duck tissues infected with Newcastle disease virus from chicken. These results suggest that free radicals may participat in the pathogenetie course of ducks infected with heterologous Newcastle disease virus.