慧星试验检测洛克沙胂对CHL细胞的DNA损伤

DNA damage induced by roxarsone in Chinese hamaster lung cells by the comet assay

利用碱性单细胞凝胶电泳技术研究洛克沙胂对中国仓鼠肺细胞(CHL)的DNA损伤影响。洛克沙胂分为10、100、500、1000mg/L剂量组,分别以磷酸盐缓冲液和10mg/L亚砷酸钠为对照组,经3、6、12、24、48h暴露后进行单细胞凝胶电泳。结果表明,慧星试验参数尾DNA含量、慧星全长、慧尾长、尾距和Olive尾距等表现出剂量-效应、时间-效应关系,不同剂量洛克沙胂、不同暴露时间下对CHL细胞有不同程度的DNA损伤。

DNA breaks induced by roxarsone in Chinese hamaster lung （CHL） cells has been studied in vitro using Alkaline single cell gel electrophoresis （comet assay）. CHL cells were exposed with doses of 0 （phosphate buffere saline）, 10,100,500,1 000 mg/L roxarsone and 10 mg/L arsenite sodium with 3,6,12,24,48 h exposure time respectively. The parameters of tail DNA, length of comet, length of tail, tail moment and Olive tail moment were measured in the comet assay. The value of these parameters enhanced with the increase of roxarsone dose and the exposure time showing the dose - effect and dose - time rules. The different level of DNA damage treated with roxarsone in CHL cells were appeared in various doses and exposure duration.