板蓝根多糖抗小鹅瘟病毒的作用

Antivirus effects of IRPS against goose parvovirus in goose embryo

在鹅胚内用板蓝根多糖(IRPS)作直接杀毒和阻断感染试验,对鹅胚尿囊液中小鹅瘟病毒(GPV)含量、鹅胚存活时间、存活率、病变和免疫组化进行测定。结果显示,40μg/胚IRPS阻断感染组,GPV接种后72h ELISA检测为阳性的尿囊液最高稀释倍数23;鹅胚平均存活时间192h;存活率6/6;胚体肉眼观察未见病变;切片镜检可见肝、肾轻度淤血和细胞变性,心、脑轻度淤血和水肿;免疫组化显示肝、肾内GPV表达抗原为弱阳性,心、脑内为阴性。而攻毒对照组尿囊液GPV阳性的最高稀释倍数28;鹅胚存活时间138h;存活率0/6;胚体严重萎缩、出血、水肿,各组织细胞碎裂、坏死,组织内GPV表达抗原全为强阳性。两者比较,各指标具有极显著差异。这表明在鹅胚内IR-PS具有极强的阻断GPV感染、增殖和保护胚体组织器官免受其损伤的作用。

To explore the antivirus effects of Isatis root polysaccharide（IRPS） against goose parvovirus（GPV）, IRPS was used directly to mix with GPV in vitro assay and then the GPV were inoculated in to the goose embryos, and both IRPS and GPV were simultaneously inoculated in to the goose embryos in vivo assay for investigate the direct killing roles and infection interruption of IRPS against GPV. The contents of GPV in allantoic fluid from goose embryos, survival time of goose embryo, survival rate, lesion and immunohistochemical assay were conducted. The result indicated the embryos in infection interruption group were injected with 40 ~tg IRPS, 72 h after inoculation by GPV, the highest dilution of allantoic fluid positive detected by ELISA was 23 , the average survival time of goose embryos was 192 h with a survival rate of 6/6 ,and no lesion was observed by naked eyes. However,histopathological examination showed slight congestion and degeneration were observed in liver and kidney cells under microscope, slight congestion and edema were also found in heart and brain tissues. The special GPV antigen in liver and kidney tissues from infection interruption group was weakly positive,and the antigen in heart and brain tissues was negative detected by immunohistochemical method. In contrast to that, the highest dilution of allantoic fluid from embryos in infection control group which inoculated with GPV was 2s ,the average survival time was 138 h with no survival embryos,and the dead embryos appeared serious atrophia, hemorrhage,edema and plasmatorrhexis,necrosis,GPV antigen in detected tissues of infection control group was all strong positive. So there were significant differences between the two groups. Conclusion：IRPS can powerfally interrupt the infection and inhibit the proliferation of GPV and protect the embryos from injury by GPV.