茶色素对胰岛素抵抗大鼠胰岛素受体的影响

Effect of Tea Pigments on Insulin Receptor in Insulin Resistant Rats

探讨茶色素对胰岛素抵抗大鼠红细胞胰岛素受体的影响。将50只清洁级SD大鼠随机分成对照组、高脂组和低中高剂量茶色素干预组。高脂组和茶色素干预组以高脂饲料饲喂8周诱发肥胖致胰岛素抵抗模型,茶色素干预组再给予0.1、0.2g/(kgbw·d)和0.4g/(kgbw·d)剂量茶色素灌胃8周,实验结束后采血测定红细胞胰岛素受体水平,同时测定血糖、血胰岛素、血FFA及血痩素。与对照组相比,高脂组空腹胰岛素、痩素和FFA明显升高(P<0.05),而红细胞高亲和力受体数目明显降低(P<0.05),与高脂组相比,中高剂量茶色素干预组空腹胰岛素、痩素和FFA明显降低(P<0.05),而红细胞高亲和力胰岛素受体数目明显升高(P<0.05)。说明茶色素干预可提高胰岛素抵抗大鼠胰岛素高亲和力受体数目。

To study the effect of theaflavins on insulin receptor in insulin-resistant rats, fifty male Sprague-Dawley rats were randomly divided into normal control group, high fat diet group and three dosed theaflavins groups, high rats of fat diet group and three dosed theaflavins groups were fed with high lipid diet for 8 weeks and then those of theaflavins groups were fed theaflavins of 0.1, 0.2 and 0.4 g/（kg hw·d） respectively for other 8 weeks. The blood serum was collected to measure insulin receptor level of red blood cell, blood glucose, free fatty acid, blood insulin and blood leptin. As compared to control group, the blood insulin, leptin and FFA of high fat diet group were higher than those of normal control group （P〈0.05）, but the number of insulin receptor was lower than that of control group （P〈0.05）. As compared to high fat diet group, the blood insulin, leptin and FFA of the middle and high dose theaftavins groups were obvious lower （P〈0.05）, the level of insulin receptor of red blood cell was higher obviously（P〈0.05）. It is considered that theaflavins can upregulate insulin receptor in insulin-resistant rats, the mechanism may relate to its down regulating blood FFA and leptin or the influence on gene regulator unit directly of receptor.