肺康颗粒对肺脾两虚型慢阻肺大鼠气道的保护作用

Airway Protective Effect of Feikang Granules on Pulmonosplenic Asthenia Rats with Chronic Obstructive Pulmonary Disease

目的观察肺康颗粒对肺脾两虚型慢性阻塞性肺病(COPD)大鼠气道重塑及D-木糖排泄率的影响。方法取SD大鼠40只,随机分为正常对照组、COPD模型组、肺康颗粒高、低剂量组(生药量13,3.25g·kg-1),每组10只。采用熏香烟、气道内多次滴入脂多糖及灌服番泻叶的方法,建立大鼠COPD肺脾两虚模型。观察大鼠一般情况;光镜下观察各组大鼠肺组织病理形态学变化;测量肺组织小气道管壁厚度;采用对溴苯胺法测定大鼠尿中D-木糖排泄率。结果与模型组相比,肺康颗粒高、低剂量组大鼠体重明显升高(P<0.05);肺泡和细支气管病理形态明显改善;大鼠肺组织小气道管壁厚度明显变薄(P<0.05);大鼠D-木糖排泄率明显增加(P<0.05)。结论肺康颗粒对肺脾两虚型慢阻肺大鼠气道重塑及D-木糖排泄率有一定的改善作用。

Objective To investigate the intervention effect of Feikang Granules （FG） on airway remodeling and D -xylose excretory rate in pulmonosplenic asthenia rats with chronic obstructive pulmonary disease （COPD）. Methods SPF SD rats were equally randomized into four groups： normal control group, COPD model control group, high -dose FG group, and low- dose FG group. The pulmonosplenic asthenia rats models with COPD were established by the way of smoking with cigarettes, repeated infusion of lipopolysaccharide in the airway and feeding with decoction of Folium Sennae. The general health state of rats was observed, and pathological changes of lung tissue were examined by the optical microscope. The wall thickness of small airway in rats was measured and the excretory rate of D - xylose was detected by bromoaniline method. Results Compared with the model control group, weight was increased, the pathological changes of pulmonary alveoli andbronchiole were relieved, small airway wall thickness became thin, and D - xylose excretory rate increased in high- dose and low- dose FG groups and the normal control group, the difference being significant （P 〈 0. 05）. Conclusion FG exerts a certain effect on airway remodeling and D - xylose excretory rate of pulmonosplenic asthenia rat with COPD.