阳春砂仁GC-MS特征指纹图谱数字化信息的GC验证

Verification of Digitized Information of GC-MS Characteristic Fingerprint of Amomum villosum by GC

目的考察阳春砂仁GC-MS特征指纹图谱数字化信息在GC中的适用性。方法分别采用GC-MS和GC分析13批次阳春砂仁的主要成分,同时用已建立的阳春砂仁挥发油特征指纹图谱数字化信息与GC-MS、GC分析的样品进行相似度比对。结果13批次阳春砂仁均含有α-蒎烯、茨烯、β-蒎烯、β-月桂烯、柠檬烯、芳樟醇、樟脑、异龙脑、龙脑、乙酸龙脑酯等10个主要特征成分,合计相对含量达(88.15±2.97)%,具有一定代表性。以此10个特征指标成分计,GC-MS分析样品与GC分析样品的相似度为0.994～1.000(夹角余弦法);与阳春砂仁GC-MS特征指纹图谱数字化信息比对,GC-MS分析样品的相似度为0.978～0.999(夹角余弦法),GC分析样品的相似度为0.986～0.998(夹角余弦法)。结论阳春砂仁GC-MS特征指纹图谱数字化信息能应用于GC中,使不同时间、不同型号的仪器、色谱柱、色谱条件下的分析结果可以相互比对,具有推广应用价值,可用于药材的质量评价与鉴定。

Objective To investigate the applicability of the digitized standard of GC - MS characteristic fingerprint in GC. Methods The GC - MS and GC were used to analyze the essential components of thirteen batches of Amomum villosum samples. The digitized standard of characteristic fingerprint of Amomum villosum essential oil was compared with the result of the samples detected by GC - MS and GC. Results Ten essential characteristic components have been identified in thirteen batches of Amomum villosum samples, and their relative content was （88.15 ±2.97）% , which are the representative components. The main components were α - pinene, camphene, β - pinene, β - myreene, limonene, linalol, camphor, isoborneol, borneol and borneol acetate. With the ten components as indexes, the sample similarity calculated by cosin method was 0. 994 - 1. 000 when analyzed by GC - MS and GC, 0. 978 - 0. 999 when analyzed by GC -MS and the digitized standard of GC -MS characteristic fingerprint, and 0. 986 -0. 998 when analyzed by GC and the digitized standard of GC - MS characteristic fingerprint. Conclusion The digitized standard of GC - MS characteristic fingerprint of Amomum villosum can be used in GC, which will ensure the comparison of results obtaining at different time by different types of machines, on different chromatographic columns and under different conditions. The method can be used for quality evaluation of Amomum villosum.