HPLC法测定黄连降糖片中葛根素的含量

Determination of Puerarin in Huanglian Jiangtang Tablets by HPLC

目的测定黄连降糖片中葛根素的含量。方法样品用30%乙醇超声提取进样,色谱柱:DiamonsilC18柱(250mm×4.6mm,5μm),流动相:乙腈-0.5%冰醋酸溶液(梯度洗脱),检测波长:250nm,流速:1mL/min。结果葛根素在0.338～2.366μg之间与峰面积线性关系良好,r=0.999997(n=7),平均回收率100.2%,RSD为1.80%(n=6)。结论该法简便,快速,准确,可用于该制剂及葛根药材的含量测定及质量控制。

Objective To establish a method for the determination of Puerarin in Huanglian Jiangtang Tablets. Methods The sample was extracted with 30 % ethanol. The chromatographic conditions were as follows： Diamonsil C18 chromatographic column （250 mm × 4.6 mm, 5 μm）with a mobile phase of acetonitrile -0.5 % glacial acetic acid, the detection wavelength being at 250 nm and the flow rate being 1.0 mL · min^-1. Results A linearity was obtained from 0. 338 μg to 2. 336μg of Puerarin in Huanglian Jiangtang Tablets with a good correlation （ r = 0. 999 997, n = 7）. The average recovery was 100.2 % and RSD = 1.80 % （ n = 6）. Conclusion This method for determination of Puerarin in Huanglian Jiangtang Tablets is easy, sensitive, specific and accurate.