鼻咽癌组织表达VCAM-1与局部淋巴细胞浸润的关系

Relation between expression of VCAM-1 and infiltrating lymphocytes in nasopharyngeal carcinoma

目的:观察鼻咽癌组织表达血管粘附分子-1(VCAM-1)与局部浸润淋巴细胞的关系。方法:收集51例鼻咽癌和22例慢性鼻咽炎石蜡标本和临床资料,观察癌巢间淋巴细胞浸润程度,计数癌巢内淋巴细胞数量;用VCAM-1、CD3、CD4、CD8单克隆抗体进行免疫组化染色,并判断鼻咽癌表达VCAM-1程度,计数CD3+和CD4+、CD8+T细胞。结果:(1)癌细胞表达VCAM-1的鼻咽癌病例达92.16%。(2)鼻咽癌细胞表达VCAM-1与淋巴结转移与否、分化程度和临床分期均无关(P>0.05)。(3)鼻咽癌细胞表达VCAM-1与巢间淋巴细胞浸润程度、巢内淋巴细胞数量、CD4+、CD8+T细胞数量均无关(P>0.05)。(4)鼻咽癌细胞轻度表达VCAM-1时组织中CD3+T淋巴细胞明显增多(P=0.009),但不显示强度依赖性作用。结论:(1)VCAM-1在鼻咽癌细胞表达率很高,其表达强度与转移和临床分期无关。(2)鼻咽癌细胞表达VCAM-1与正常细胞表达VCAM-1的生理作用有所不同,不显示明确地吸引淋巴细胞的作用。

Objective： To observe the relation between expression of vascular cell adhesin molecule-1 （VCAM-1） on nasopharyngeal carcinoma and infiltrating lymphocytes. Methods： The lymphocytes infiltrating in stroma and in carcinoma nest were observed on paraffin slides coming from 51 cases with nasopharyngeal carcinoma （NPC）. Meanwhile the expression of VCAM-1 and the number of T lymphocytes and CD^3＋ CD^4＋ and CD^8＋ sub- set of T lymphocytes were counted on immunochemical staining slides. Results： （ 1 ） 92.16% NPC cases （47/ 51 ） expressed the VCAM-1 at different degrees. （2） No relation existed between expression of VCAM-1 and either node metastasis, tumor differentiation and stage, or infiltrating lymphocytes, CD^4＋ and CD^8＋ T cell subsets. （3） Weak expression of VCAM-1 by nasopharyngeal carcinoma cell correlated with increased number of CD^3＋ T cell（ P = 0.009）, but there was no intensity dependent relation between two events. Conclusion： （ 1 ） VCAM-1 highly expressed on NPC but independently of the metastasis, tumor differentiation and stages. （2） The VCAM- 1expressed by NPC cells did not show definite effect of attracting the lymphocytes as that by normal ceUs did.