摘要
目的:建立HPLC同时测定姜黄中姜黄素、脱甲氧基姜黄素和双脱氧基姜黄素含量的测定方法,并对贵州(野生、种植)和全国其他不同产地姜黄中的3种姜黄素进行含量测定,为姜黄的质量控制提供参考。方法:采用Hypersil ODS_2柱(250mm×4.6 nm,5μmn),流动相为乙腈-0.5%醋酸水溶液(44:56),流速1.0 mL min^(-1),检测波长425 nm,柱温35℃。结果:姜黄素、脱甲氧基姜黄素、双脱甲氧基姜黄素的线性范围分别为0.001~1.59μg(r=0.9998),0.001~1.49μg(r=0.9999),0.001~1.55μg(r=0.9999);平均回收率(n=9)分别为98.2%,97.4%,98.0%。结论:不同产地的姜黄中姜黄素。
Objective: To establish an HPLC method for simultaneous determination of curcumin, demethoxycurcumin and bidemethoxycurcumin of Curcuma longa L. The components of 33 raw herbs of Curcuma longa L. from different places of China were investigated. Method:An Hypersil ODS2 column (250 mm × 4.6 mm,5 μm), with acetonitrile-0. 5% acetic acid(44:56)as mobile phase. The flow rate was 1.0 mL ·min^-1 and column temperature was maintained at 35 ℃. The detection wavelength was set at 425 nm for acquiring chromatograms. Results:The calibration curves of curcumin,demethoxycurcumin and bidemethoxycurcumin were linear in the range of 0. 001 - 1.59 μg( r = 0. 9998 ) ,0. 001 - 1.49 μg (r = 0. 9999 ) ,0. 001 - 1.55 μg( r = 0. 9999 ), respectively;The average recoveries( n = 9) were 98.2% ,97.4% and 98.0%, respectively. Conclusion:The very important findings are that curcumin, demethoxycurcumin and bidemethoxycurcumin of Curcuma longa L. will vary greatly in different locations.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第11期1810-1814,共5页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金2007年资助项目[No.30760293]
贵阳市2006年科学技术计划项目[2006-42]