反相高效液相色谱法同时测定酚氨咖敏颗粒中四组分的含量

Simultaneous RP-HPLC determination of four components in paracetamol,caffeine,aminophenazone and chlorhenamine maleate granules

目的:建立测定酚氨咖敏颗粒中四组分的分析方法。方法:反相高效液相色谱法,采用Diamonsal C_(18)柱(150 mm×4.6mm,5μnm),以甲醇-0.1%二乙胺(25:75)(用冰醋酸调pH至4.5)为流动相;检测波长:对乙酰氨基酚、咖啡因、氨基比林为275nm,马来酸氯苯那敏为215 nm;流速:1.0 mL·min^(-1)。结果:对乙酰氨基酚、咖啡因、氨基比林、马来酸氯苯那敏线性范围分别为25 400,6～120,20～320,3～50μg·mL^(-1),平均回收率(n=9)分别为99.35%,100.10%,101.20%,101.92%。结论:该方法分离度良好,灵敏度高,准确且简便易行。

Objective ：To establish a method for determination of four components in paracetamol, caffeine, aminophenazonc and chlorhenamine maleate granules. Methods：The RP- HPLC system consisted of Diamonsal C18 （150 mm × 4.6 mm,5 μm） column, with methanol - 0. 1% diethylamine （ 25： 75 ） as mobile phase （ adjusted pH to 4. 5 with glacial acetic acid）, the UV detector wavelength was 275 nm for paracetamol, caffeine and aminophenazone, 215 nm for chlorhenamine maleate,the flow rate was 1.0 mL ·min^-1. Results：The linear ranges were 25 -400 μg·mL^-1 for paracetamol, 6 - 120μg·mL^-1 for caffeine, 20 -320 μg·mL^-1 for aminophenazone and 3 -50μg·mL^-1 for chlorhenamine maleate respectively. The average recoveries （n = 9）were 99.35% for paracetamol, 100. 10% for caffeine, 101.20% for aminophenazone and 101.92 % for chlorhenamine maleate. Conclusions： The proposed method was sensitive, accurate and simple with good resolution.