大孔树脂-凝胶柱层析法大规模制备纯品羟基红花黄色素A

Large scale preparation of pure Hydroxysafflor Yellow A by macroporus resin-gel column chromatography

目的:以大孔树脂-凝胶柱层析法制备羟基红花黄色素A(HSYA)。方法:室温浸提法提取红花水提液,大孔树脂柱层析法制备HSYA粗品,Sephadex LH-20凝胶柱层析法制备HSYA纯品,HPLC法分析HSYA纯品纯度,观察该HSYA抑制ADP诱导家兔体外血小板聚集的作用。结果:红花水提液经大孔树脂-凝胶柱层析法获得HSYA的纯度为95.3%,该成分抑制ADP诱发的家兔血小板聚集呈量效关系。结论:大孔树脂-凝胶柱层析法制备HSYA的方法为一简便、有效的技术。

Objective： Preparing Hydroxysafflor Yellow A （HSYA） by macroporus resin-gel column chromatography. Method：Aqueous extract was prepared from Carthamus tinctorius L.. Crude HSYA was prepared by macroporus resin chromatography and purified HSYA was further prepared by Sephadex LH-20 column chromatography. The purity was analyzed by HPLC. The inhibition of HSYA on rabbit platelet aggregation induced by ADP was observed. Result： From aqueous extract HSYA was prepared through macroporus resin-gel column chromatographic procedure and its purity was 95.3 % . This ingredient was active to inhibit platelet aggregation induced by ADP. Conclusion： HSYA preparation by macroporus resin-gel chromatography is a simple and effective technology.